62 1e6 anti snail2 Search Results


snail2 slug  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank snail2 slug
Snail2 Slug, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
snail2 slug - by Bioz Stars, 2026-03
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anti snail2  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank anti snail2
(A) The three VICKZ paralogs were detected by RT-PCR in isolates from chick embryos at the 4–10 somite-stage (lanes 1–4) and at E2.5 (trunk region, lanes 5–9), using primer sets for cVICKZ3 (lanes 1,5), cVICKZ2 (lanes 2,6) and cVICKZ1 (lanes 3,7). All RT-PCR products were the appropriate size on ethidium bromide stained agarose-gel (cVICKZ3 = 426nt, cVICKZ2 = 206nt, cVICKZ1 = 172nt). Marker-1kb ladder. Negative controls: cVICKZ3-RT, PCR reaction using cVICKZ3 primers but without Reverse Transcriptase (lanes 4,8). Positive controls: cVICKZ3 (clone 5e15, Pubmed accession number AJ719686; lanes 9–11) and ZBP-1 for cVICKZ1 (lanes 12–14) using primer sets for cVICKZ3 (lanes 9,12), cVICKZ2 (lanes 10,13) and cVICKZ1 (lanes 11,14). (B) Proteins were extracted from tailbud stage Xenopus embryos, or from chick E2 stage whole embryos, head, or trunk. One embryo equivalent was loaded on each lane, and VICKZ expression was examined following electrophoresis by western blot analysis using an anti-pan VICKZ antibody. (C-K) Cross-sections at the midbrain (MB) level in chick embryos at 5ss (C,F,H,J), 8ss (D,G,I,K), and 13ss (E) were stained using antibodies directed against VICKZ (C-G, J-K), HNK-1 (E), <t>Snail2</t> (F-G), ZO-1 (H-I), and N-cadherin (J-K). (F’) and (G’) are higher magnifications of the individual channels (red, green, and merge) of the boxed regions in (F) and (G). At early stages of CNC specification, presumptive CNC cells are located in the neural folds in the dorsolateral region (C, F, F’, H, J, arrows), where they express both VICKZ and Snail2 (F). ZO-1 and N-cadherin are expressed along the apical surface of the neuroepithelium at this stage, but are mostly excluded from the neural folds (H,J). Down-regulation of VICKZ occurs in 8ss embryos in the dorsal-most, delaminating CNC cells that remain Snail2-positive (G, arrow, G’) and are negative for ZO-1 and N-cadherin (I,K arrowhead). HNK-1-positive migrating CNC do not express VICKZ (E, arrows). Abbreviations: MB, midbrain. All sections were also stained with Hoechst to show nuclei (blue).
Anti Snail2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti snail2/product/Developmental Studies Hybridoma Bank
Average 94 stars, based on 1 article reviews
anti snail2 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier

Image Search Results


(A) The three VICKZ paralogs were detected by RT-PCR in isolates from chick embryos at the 4–10 somite-stage (lanes 1–4) and at E2.5 (trunk region, lanes 5–9), using primer sets for cVICKZ3 (lanes 1,5), cVICKZ2 (lanes 2,6) and cVICKZ1 (lanes 3,7). All RT-PCR products were the appropriate size on ethidium bromide stained agarose-gel (cVICKZ3 = 426nt, cVICKZ2 = 206nt, cVICKZ1 = 172nt). Marker-1kb ladder. Negative controls: cVICKZ3-RT, PCR reaction using cVICKZ3 primers but without Reverse Transcriptase (lanes 4,8). Positive controls: cVICKZ3 (clone 5e15, Pubmed accession number AJ719686; lanes 9–11) and ZBP-1 for cVICKZ1 (lanes 12–14) using primer sets for cVICKZ3 (lanes 9,12), cVICKZ2 (lanes 10,13) and cVICKZ1 (lanes 11,14). (B) Proteins were extracted from tailbud stage Xenopus embryos, or from chick E2 stage whole embryos, head, or trunk. One embryo equivalent was loaded on each lane, and VICKZ expression was examined following electrophoresis by western blot analysis using an anti-pan VICKZ antibody. (C-K) Cross-sections at the midbrain (MB) level in chick embryos at 5ss (C,F,H,J), 8ss (D,G,I,K), and 13ss (E) were stained using antibodies directed against VICKZ (C-G, J-K), HNK-1 (E), Snail2 (F-G), ZO-1 (H-I), and N-cadherin (J-K). (F’) and (G’) are higher magnifications of the individual channels (red, green, and merge) of the boxed regions in (F) and (G). At early stages of CNC specification, presumptive CNC cells are located in the neural folds in the dorsolateral region (C, F, F’, H, J, arrows), where they express both VICKZ and Snail2 (F). ZO-1 and N-cadherin are expressed along the apical surface of the neuroepithelium at this stage, but are mostly excluded from the neural folds (H,J). Down-regulation of VICKZ occurs in 8ss embryos in the dorsal-most, delaminating CNC cells that remain Snail2-positive (G, arrow, G’) and are negative for ZO-1 and N-cadherin (I,K arrowhead). HNK-1-positive migrating CNC do not express VICKZ (E, arrows). Abbreviations: MB, midbrain. All sections were also stained with Hoechst to show nuclei (blue).

Journal: PLoS ONE

Article Title: A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis

doi: 10.1371/journal.pone.0136408

Figure Lengend Snippet: (A) The three VICKZ paralogs were detected by RT-PCR in isolates from chick embryos at the 4–10 somite-stage (lanes 1–4) and at E2.5 (trunk region, lanes 5–9), using primer sets for cVICKZ3 (lanes 1,5), cVICKZ2 (lanes 2,6) and cVICKZ1 (lanes 3,7). All RT-PCR products were the appropriate size on ethidium bromide stained agarose-gel (cVICKZ3 = 426nt, cVICKZ2 = 206nt, cVICKZ1 = 172nt). Marker-1kb ladder. Negative controls: cVICKZ3-RT, PCR reaction using cVICKZ3 primers but without Reverse Transcriptase (lanes 4,8). Positive controls: cVICKZ3 (clone 5e15, Pubmed accession number AJ719686; lanes 9–11) and ZBP-1 for cVICKZ1 (lanes 12–14) using primer sets for cVICKZ3 (lanes 9,12), cVICKZ2 (lanes 10,13) and cVICKZ1 (lanes 11,14). (B) Proteins were extracted from tailbud stage Xenopus embryos, or from chick E2 stage whole embryos, head, or trunk. One embryo equivalent was loaded on each lane, and VICKZ expression was examined following electrophoresis by western blot analysis using an anti-pan VICKZ antibody. (C-K) Cross-sections at the midbrain (MB) level in chick embryos at 5ss (C,F,H,J), 8ss (D,G,I,K), and 13ss (E) were stained using antibodies directed against VICKZ (C-G, J-K), HNK-1 (E), Snail2 (F-G), ZO-1 (H-I), and N-cadherin (J-K). (F’) and (G’) are higher magnifications of the individual channels (red, green, and merge) of the boxed regions in (F) and (G). At early stages of CNC specification, presumptive CNC cells are located in the neural folds in the dorsolateral region (C, F, F’, H, J, arrows), where they express both VICKZ and Snail2 (F). ZO-1 and N-cadherin are expressed along the apical surface of the neuroepithelium at this stage, but are mostly excluded from the neural folds (H,J). Down-regulation of VICKZ occurs in 8ss embryos in the dorsal-most, delaminating CNC cells that remain Snail2-positive (G, arrow, G’) and are negative for ZO-1 and N-cadherin (I,K arrowhead). HNK-1-positive migrating CNC do not express VICKZ (E, arrows). Abbreviations: MB, midbrain. All sections were also stained with Hoechst to show nuclei (blue).

Article Snippet: Antibodies used were: anti-Desmin and anti-GFP (Molecular Probes), N-cadherin and ZO-1 (Zymed), anti-integrin α6 and anti-snail2 (P2C62C4 and 62.1E6, Developmental Studies Hybridoma Bank), affinity purified anti-panVICKZ [ , ] and HNK-1 (BD Pharmingen San-Diego, CA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Marker, Reverse Transcription, Expressing, Electrophoresis, Western Blot